Agilent ACEA NovoCyte 3000 Flow Cytometer w/ NovoSampler Pro & Fluidics Station

Description

Agilent ACEA NovoCyte 3000 Flow Cytometer w/ NovoSampler Pro & Fluidics Station

Included in This Purchase: 

• Acea NovoCyte 3000 
• Agilent NovaSampler Pro 
• Fluidics Cart and Harness 
• NovoRinse, NovoClean, NovoFlow, and Waste caps and bottles. 
• Desktop PC (monitor/keyboard/mouse) running NovoExpress V1.6.2 
• Communication and power cables

Instrument Application: 

• Immunophenotyping, Cell cycle analysis, Apoptosis detection, Multiplexed cytokine analysis, rare cell population detection

NovoCyte 3000 Flow Cytometer Configuration: 

• Optics:(3) Lasers, (13) Colors, (15) Detectors: 
• Forward Scatter Channel: 488 blue laser 
• Side Scatter Channel: 488 blue laser 
• 405nm Violet Laser: 780/60, 675/30, 615/20, 572/28, 530/30, 445/45. 
• 488nm Blue Laser: 780/60, 675/30, 615/20, 572/28, 530/30. 
• 640nm Red Laser: 780/60, 675/30. 
• Laser Color Allocation (Laser/Channel Count): Violet/6, Blue/5 and Red/2 
• Scatter resolution: 0.2 um 
• Cell size: 0.2 to 50 µm 
• Fluorescence threshold sensitivity: FITC <75 MESF, PE <50 MESF, APC <20 MESF 
• Fluorescence resolution: <3% CV for CEN

NovoSampler Pro NS200  

• Compatibility: Supports 24-, 48-, and 96-well plates, as well as 24 tube racks.  
• Carryover: Low carryover of <0.1% (volume).  
• Sample Mixing: Orbital shaking up to 3000 rpm for consistent sample mixing.  
• Automation: Walk-away convenience for time and cost savings.  
• Software Integration: Controlled via NovoExpress software.  
• Applications: Suitable for various assays, including apoptosis and cell cycle analysis.

Fluidics 

• • Sample acquisition rate: 35,000 events/second 
  • Volumetric absolute count precision: CV <5% 
  • Sample flow rate: 5 to 120 μL/min 
  • Sheath flow rate: 6.5 mL/min 
  • Sample aspiration volume: 10 μL to 5 mL 
  • Fluid container capacity: 3 L sheath, 3 L waste, 500 mL cleaning, 500 mL decontamination 
  • Carryover: <0.1% 
  • Manual sample loading: 12 x 75 mm tube, 1.5 mL Eppendorf tube 
  • Automatic sample loading with NovaSampler Pro: standard 96-well plates (flat-bottom, U-bottom, V-bottom), standard 24-well and 48-well plates, and ACEA NovoSampler Pro 24-tube rack for 12 × 75 mm tubes 
  • Power requirements: input: 12 VDC, power: 90 VA 

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