Description
BioTek Cytation 5 Cell Imaging Microplate Reader & EONC Microplate Reader
These readers are in very good condition. Retrieved from working Lab. These readers are not used together as a system, but as 2 units that are controlled via the same software. Program lets user select which reader will be used for research being performed.
The Agilent BioTek Cytation 5 CYT5FA is a powerful, modular hybrid instrument that combines automated digital widefield microscopy with a conventional multi-mode microplate reader. Designed for life science and drug discovery research, it allows labs to capture both phenotypic cellular images and quantitative plate data in a single run.
The BioTek EONC (part of the Agilent BioTek Eon line) is a high-performance, monochromator-based microplate spectrophotometer equipped with a dedicated cuvette port. It is primarily used in life science and clinical laboratories for rapid, filter-free absorbance readings, endpoint assays, and spectral scanning.
Cytation 5 – CYT5FA
- Magnification: 1.25x up to 60x
- Objectives: 6-position automated turret supporting Olympus or Zeiss user-replaceable objectives
- Light Source / Filters: High-power LEDs with user-replaceable fluorescence cubes (plus dedicated brightfield channel)
- Camera: Monochrome or color CCD digital camera
- Imaging Modes: Fluorescence, brightfield, high-contrast brightfield, and digital phase contrast
- Patented Hybrid Optics: Combines filter-based optics (high sensitivity/transmission) and monochromator-based optics (assay flexibility)
- Wavelength Selection: Quadruple monochromators (top/bottom) and deep blocking bandpass filters
- Wavelength Range: Fluorescence (300 to 700 nm), UV-Vis Absorbance (200 to 999 nm)
- Detection Modes: Fluorescence Intensity, Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP), Luminescence, UV-Vis Absorbance, and Alpha assays
- Microplate Compatibility: 6- to 384-well plates for monochromators; 6- to 1536-well plates for filter-based and imaging assays
- Other Labware: Microscope slides, Petri dishes, cell culture dishes, T25 flasks, and 2 μL Take3 micro-volume plates
- Automation: Fully compatible with Agilent BioTek BioSpa 8 automated incubator and BioStack microplate stacker (not included)
- Incubation: 4-zone incubation up to 65°C with active condensation control; ± 0.2°C uniformity at 37°C
- Shaking: Linear, orbital, and double-orbital shaking
- Power: Max 250W power consumption
- Weight: roughly 80 lbs (36.3 kg)
Microplate Reader – EONC
- Wavelength Range: 200–999 nm, selectable in 1 nm increments.
- Plate Compatibility: Reads 6- to 384-well microplates.
- Cuvette Port: Allows for quick, traditional cuvette-based assays without tying up a plate.
- Temperature Control: 4-Zone incubation up to 65 °C to support temperature-sensitive assays.
- Shaking: Linear, orbital, and double orbital shake modes.
- Software Integration: Driven by Agilent BioTek Gen5 Software for protocol management, data analysis, and exporting.
- Micro-Volume Ready: Compatible with BioTek Take3 Plates for nucleic acid and protein quantification using just 2 μL of sample.
- Applications: ELISA and cytotoxicity studies ~ DNA, RNA, and protein quantification ~ Enzyme kinetics and cell proliferation assays
