Description
Eppendorf Dual-Head ICSI / Transgenic Microinjection Rig
Item is in good condition. All operational testing was completed successfully, and unit functions as intended. Sold with everything pictured.
Eppendorf FemtoJet (Model 5247)
- Electronic programmable microinjector with integrated low-noise compressor for microinjection of liquids into suspension or adherent cells.
- Model Number: 5247
- Electrical Ratings: 100–240 V AC, 50–60 Hz, <0.5 A, 50 W, Fuse T 1.0 A / 250 V
- Injection Pressure (pi): 0 to 6,000 hPa (approx. 0 to 87.0 psi / 6.0 bar), adjustable in 1 hPa increments
- Compensation Pressure (pc): 0 to 6,000 hPa (approx. 0 to 87.0 psi) to prevent capillary backflow
- Clean / Rinsing Pressure: Up to 7,000 hPa (102 psi) for clearing capillary clogs
- Injection Time (ti): 0.1 to 99.9 seconds (or continuous manual mode)
- Injection Volume Range: Femtoliters (fL) to microliters (uL)
- Interfaces: 9-pin sub-D trigger port (for hand/foot switch), 15-pin interface port for synchronization with Eppendorf micromanipulators
Eppendorf TransferMan NK 2 Micromanipulators (Two Units: Models 5181 & 5188)
- High-precision motorized 3-axis micromanipulators with dynamic proportional joystick control for positioning holding and injection micropipettes.
- System A (Model 5181)
- Serial Number: 5181AN303243 (matched control base & motor drive head)
- Control Base Input Rating: 15–18 V DC, 1.6 A, 25 W (requires power supply 5181 610.001, included)
- System B (Model 5188)
- Serial Number: 5188AQ005547 (matched control base & motor drive head)
- Control Base Input Rating: 15–18 V DC, 1.6 A, 25 W (requires power supply 5181 610.001, included)
- TransferMan NK 2 Operational Specs (Both Units):
- Travel Range: > 20 mm per axis (X, Y, Z)
- Step Resolution: < 40 nm per micro-step
- Maximum Drive Speed: approx. 7.5 mm/s
- Control Modes: Proportional fine/coarse movement via central dual-action joystick, automated axial injection movement, and programmable speed dials
- Memory Positions: Storage and recall for up to 3 independent coordinate sets (e.g., cell search, injection level, home/tool-exchange position)
- Z-Limit Setting: Electronic lower-limit threshold to prevent microcapillary breakage against dish bottoms
Eppendorf CellTram Air (Model 5176)
- Manual pneumatic piston microinjector/aspirator, primarily used to hold oocytes, embryos, or suspension cells via controlled negative pressure.
- Model Number 5176
- Pressure Generation: Maintenance-free, air-filled cylinder/piston assembly
- Maximum Output Pressure: 2,900 hPa (approx. 42 psi / 2.9 bar)
- Volume Displacement per Revolution: 88 uL per full dial turn
- Total Displacement Volume: 2,640 uL (15 mm total piston travel / 30 turns)
- Minimum Adjustable Volume: < 200 nL
- Mounting: Heavy non-slip cast-iron base plate with ergonomic coarse adjustment dial
Supporting Hardware & Accessories On Hand
- Power Supplies (2 units): Both external AC/DC desktop adapters (5181 610.001, 100–240 V AC input to 15–18 V DC output).
- Mounting Towers: Two heavy vertical mounting posts with sliding brackets for the 5181/5188 motor drive units.
- Stage / Condenser Insert: Circular microscope stage adapter plate with cutout ring.
- Cables & Hardware: Motor-to-console ribbon/data cables, RS-232/D-sub interconnect cables, horizontal mounting rods, clamping screws, and small installation tool bags.
- Documentation: Original printed Eppendorf TransferMan NK 2 user manual.
