Description
Agilent ACEA NovoCyte 3000 Flow Cytometer w/ NovoSampler Pro & Fluidics Station
Included in This Purchase:
- Acea NovoCyte 3000
- Agilent NovaSampler Pro
- Fluidics Cart and Harness
- NovoRinse, NovoClean, NovoFlow, and Waste caps and bottles.
- Desktop PC (monitor/keyboard/mouse) running NovoExpress V1.6.2
- Communication and power cables
Instrument Application:
- Immunophenotyping, Cell cycle analysis, Apoptosis detection, Multiplexed cytokine analysis, rare cell population detection
NovoCyte 3000 Flow Cytometer Configuration:
- Optics:(3) Lasers, (13) Colors, (15) Detectors:
- Forward Scatter Channel: 488 blue laser
- Side Scatter Channel: 488 blue laser
- 405nm Violet Laser: 780/60, 675/30, 615/20, 572/28, 530/30, 445/45.
- 488nm Blue Laser: 780/60, 675/30, 615/20, 572/28, 530/30.
- 640nm Red Laser: 780/60, 675/30.
- Laser Color Allocation (Laser/Channel Count): Violet/6, Blue/5 and Red/2
- Scatter resolution: 0.2 um
- Cell size: 0.2 to 50 µm
- Fluorescence threshold sensitivity: FITC <75 MESF, PE <50 MESF, APC <20 MESF
- Fluorescence resolution: <3% CV for CEN
NovoSampler Pro NS200
- Compatibility: Supports 24-, 48-, and 96-well plates, as well as 24 tube racks.
- Carryover: Low carryover of <0.1% (volume).
- Sample Mixing: Orbital shaking up to 3000 rpm for consistent sample mixing.
- Automation: Walk-away convenience for time and cost savings.
- Software Integration: Controlled via NovoExpress software.
- Applications: Suitable for various assays, including apoptosis and cell cycle analysis.
Fluidics
-
- Sample acquisition rate: 35,000 events/second
- Volumetric absolute count precision: CV <5%
- Sample flow rate: 5 to 120 μL/min
- Sheath flow rate: 6.5 mL/min
- Sample aspiration volume: 10 μL to 5 mL
- Fluid container capacity: 3 L sheath, 3 L waste, 500 mL cleaning, 500 mL decontamination
- Carryover: <0.1%
- Manual sample loading: 12 x 75 mm tube, 1.5 mL Eppendorf tube
- Automatic sample loading with NovaSampler Pro: standard 96-well plates (flat-bottom, U-bottom, V-bottom), standard 24-well and 48-well plates, and ACEA NovoSampler Pro 24-tube rack for 12 × 75 mm tubes
- Power requirements: input: 12 VDC, power: 90 VA
